Spatial distribution of tissue fluorescence

Ivan S. Melnik; Sergiy M. Dets; Nikolay A. Denisov; Alexander M. Ovcharuk; Alexander Y. Joffe

doi:10.1117/12.237586

5 April 1996 Spatial distribution of tissue fluorescence

Ivan S. Melnik, Sergiy M. Dets, Nikolay A. Denisov, Alexander M. Ovcharuk, Alexander Y. Joffe

Proceedings Volume 2679, Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases III: Optical Biopsy; (1996) https://doi.org/10.1117/12.237586
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

Fluorescence signal during tissue LIF-analysis depends on both excitation conditions and tissue optics, registration optics and location of the probe relative to tissue sample as well. To develop reliable fiber optic probes and optimize their position the spatial distribution of tissue fluorescence should be studied. Fluorescence indicatrices of skin of rat were measured in angular range of 80 degrees. Excited light from He-Cd (20 mW, 442 nm) laser was delivered on the cutaneous surface at the angular range from 0 to 60 degrees with the tissue surface. Fluorescence was registered in the spectrum between 530 nm and 700 nm with 1.5 nm resolution. Autofluorescence of the skin of 5 white rats was studied in-vivo. Local application of sensitizer hypericin was used for stimulated fluorescence studies. Fluorescence indicatrices were not corresponded to scattering ones under the same conditions and depended on incident angle of excitative laser beam. No influence of polarization of excitative beam on outside fluorescence distribution was observed. Maximum in-vivo fluorescence yield was registered at the normal incidence. There were observed marked differences between spatial distribution of normal and photosensitized rat skin tissues.

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Ivan S. Melnik, Sergiy M. Dets, Nikolay A. Denisov, Alexander M. Ovcharuk, and Alexander Y. Joffe "Spatial distribution of tissue fluorescence", Proc. SPIE 2679, Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases III: Optical Biopsy, (5 April 1996); https://doi.org/10.1117/12.237586

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KEYWORDS

Luminescence

Skin

Tissue optics

Tissues

In vivo imaging

Laser induced fluorescence

Sensors

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