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1 April 1996 Design of separation media for DNA sequencing in capillaries
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Abstract
Practical DNA sequencing in a capillary format requires sieving fluids that can be replaced after each sequencing experiment. Uncrosslinked water soluble polymers were used to prepare DNA sieving networks, both through simple entanglements of linear polymers, and by end- capping with hydrophobic tails; aqueous solutions of the end-capped polymers yield viscous networks held together by micellar crosslinks. Both networks are effective resolving media for DNA sequencing ladders, and flow under stress, which has enabled the design of an automated capillary DNA sequencing system composed of a gel pump interfaced to an electrophoresis apparatus. The sieving properties of these polymers are described.
© (1996) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Steve Menchen, Ben A. Johnson, Ramki Madabhushi, and Mitchell A. Winnik "Design of separation media for DNA sequencing in capillaries", Proc. SPIE 2680, Ultrasensitive Biochemical Diagnostics, (1 April 1996); https://doi.org/10.1117/12.237618
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