A general technique linking micro-scale affinity capture with matrix-assisted laser desorption/ionization (MALDI) mass spectrometric detection has been developed for the rapid, sensitive and accurate determination of antigens present in biological fluids. Strategies for the qualitative and quantitative determination of single and/or multiple antigens are presented.
Jennifer R. Krone,
Randall W. Nelson,
Peter W. Williams,
"Mass spectrometric immunoassay", Proc. SPIE 2680, Ultrasensitive Biochemical Diagnostics, (1 April 1996); doi: 10.1117/12.237632; https://doi.org/10.1117/12.237632