1 April 1996 Polarized scattered light as a probe for structure and change in bioparticles
Author Affiliations +
Proceedings Volume 2680, Ultrasensitive Biochemical Diagnostics; (1996); doi: 10.1117/12.237598
Event: Photonics West '96, 1996, San Jose, CA, United States
Abstract
Polarized light scattered from small particles -- spheres, fibers, dust, defects and biomaterial -- carries information about the optical and geometrical properties and even more esoteric properties of the scatterer. This highly developed, powerful, but under used technique is sensitive enough to detect a 0.5 nanometer radius change in a 1.0 micron diameter quartz fiber. The complete set of 16 polarized light scattering signals from any scatterer form a 16- element Mueller matrix, Sij, which is the signature of the scatterer's status quo. Changes in cell morphology, internal arrangement and metabolic activity cause changes in radius, refractive index and absorption and therefore changes in the polarization signals Sij which act as the probe. We demonstrate how the Sij can type human red blood cells, do bacteria autopsies, distinguish between different types of white blood cells, bacteria, spores, and study cell life cycle and degradation as biomaterial undergoes biologically significant change.
© (1996) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
William S. Bickel, Mary E. Stafford, "Polarized scattered light as a probe for structure and change in bioparticles", Proc. SPIE 2680, Ultrasensitive Biochemical Diagnostics, (1 April 1996); doi: 10.1117/12.237598; https://doi.org/10.1117/12.237598
PROCEEDINGS
12 PAGES


SHARE
KEYWORDS
Light scattering

Particles

Polarization

Scattering

Blood

Bacteria

Optical components

Back to Top