Rapid protein characterization using bioreactive mass spectrometer probe tips

Randall W. Nelson; J. Kathleen Lewis; David Dogruel; Jennifer R. Krone; Peter W. Williams

doi:10.1117/12.237628

1 April 1996 Rapid protein characterization using bioreactive mass spectrometer probe tips

Randall W. Nelson, J. Kathleen Lewis, David Dogruel, Jennifer R. Krone, Peter W. Williams

Proceedings Volume 2680, Ultrasensitive Biochemical Diagnostics; (1996) https://doi.org/10.1117/12.237628
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

Methods have been developed for the rapid and sensitive mass spectrometric characterization of peptides. The approach uses bioreactive mass spectrometer probe tips which are capable of modifying proteins for analytical purpose. In the demonstrated case, enzymatic proteolysis is initiated upon application of analyte to the probe tips, time allowed for digestion, and the results analyzed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The probe tips have been used for proteolytic mapping and partial sequence determination of picomol quantities of peptide while maintaining analysis times of approximately 5 minutes.

Citation Download Citation

Randall W. Nelson, J. Kathleen Lewis, David Dogruel, Jennifer R. Krone, and Peter W. Williams "Rapid protein characterization using bioreactive mass spectrometer probe tips", Proc. SPIE 2680, Ultrasensitive Biochemical Diagnostics, (1 April 1996); https://doi.org/10.1117/12.237628

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