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7 May 1996 Internal absorption coefficient and threshold for pulsed laser disruption of melanosomes isolated from retinal pigment epithelium
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Abstract
This study determine the threshold radiant exposure from a 10-ns pulsed laser (532 nm) which caused bovine melanosomes to present various observable endpoints of disruption. The endpoints tested were (1) a visible region of clearing in a uniform field of melanosomes under a glass cover slip, (2) an audible sound, and (3) the increase in melanin photoreactivity due to photodisruption of melanosomes. The thresholds were tested for different starting temperatures by pre-equilibrating the melanosomes in aqueous solution at various temperatures. Lower temperatures required larger exposures to attain a given endpoint. The data suggest that bovine RPE melanosomes are about 4-fold denser in melanin content than cutaneous melanosomes.
© (1996) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Steven L. Jacques, Randolph D. Glickman, and Jon A. Schwartz "Internal absorption coefficient and threshold for pulsed laser disruption of melanosomes isolated from retinal pigment epithelium", Proc. SPIE 2681, Laser-Tissue Interaction VII, (7 May 1996); https://doi.org/10.1117/12.239608
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