Bioconjugates of photosensitizers relevant for PDT

N. A. Golubeva; Marina V. Demcheva; Elena B. Chernyaeva; Raisa I. Yakubovskaya; G. M. Suhin; Gelii V. Ponomarev; Maria G. Galpern; Eugeny A. Lukyanets; Alexander P. Savitsky

doi:10.1117/12.229485

8 January 1996 Bioconjugates of photosensitizers relevant for PDT

N. A. Golubeva, Marina V. Demcheva, Elena B. Chernyaeva, Raisa I. Yakubovskaya, G. M. Suhin, Gelii V. Ponomarev, Maria G. Galpern, Eugeny A. Lukyanets, Alexander P. Savitsky

Author Affiliations +

Proceedings Volume 2728, CIS Selected Papers: Laser Use in Oncology; (1996) https://doi.org/10.1117/12.229485
Event: CIS Selected Papers: Laser Use in Oncology, 1995, -, Russian Federation

Abstract

The photodynamic therapy is highly promising for its dual specificity, namely, for the site of accumulation of the phototoxic substance and for the site of action of laser. A drawback of the PDT technique is a low selectivity of the photosensitizers accumulation in tumor tissue. Therapeutic doses of photosensitizers are fairly high, that leads to unfavorable effects (toxicoses, lesions of normal skin, prolonged rehabilitation). This drawback can be overcome is photoimmunotoxins are used, i.e. conjugates of photosensitizers with antibodies to specific tumor markers. We tried to introduce a simple model for estimation of singlet oxygen effect on cells for the case of free photosensitizers, presented in outside solution, and in case of conjugated dye, bound by cell surface receptors. To achieve the same effect the required concentration of conjugated photosensitizer can be significantly decreased (50 to 100 fold) as compared with free dye. Methods for porphyrins and different phthalocyanines and naphthalocyanines conjugation with protein were developed. The use of bacteriophage T4 as a model for studying the action of photoimmunotoxines on virus, unambiguously demonstrated the applicability of this approach for targeted destruction of viral particles.

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N. A. Golubeva, Marina V. Demcheva, Elena B. Chernyaeva, Raisa I. Yakubovskaya, G. M. Suhin, Gelii V. Ponomarev, Maria G. Galpern, Eugeny A. Lukyanets, and Alexander P. Savitsky "Bioconjugates of photosensitizers relevant for PDT", Proc. SPIE 2728, CIS Selected Papers: Laser Use in Oncology, (8 January 1996); https://doi.org/10.1117/12.229485

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