Compact phase resolved fluorescence spectrometer for detection and discrimination of airborne and waterborne bacteria

Robert C. Chin; Michael W. Mayo; William Halliday; Noe Salazar; Clay Cruce; James P. Chambers; James J. Valdes

doi:10.1117/12.260589

10 December 1996 Compact phase-resolved fluorescence spectrometer for detection and discrimination of airborne and waterborne bacteria

Robert C. Chin, Michael W. Mayo, William Halliday, Noe Salazar, Clay Cruce, James P. Chambers, James J. Valdes

Author Affiliations +

Proceedings Volume 2836, Chemical, Biochemical, and Environmental Fiber Sensors VIII; (1996) https://doi.org/10.1117/12.260589
Event: SPIE's 1996 International Symposium on Optical Science, Engineering, and Instrumentation, 1996, Denver, CO, United States

Abstract

We report on the development of a compact, portable fluorescence lifetime instrument based on phase resolved fluorescence spectroscopy (PRFS). The PRFS biosensor has been designed for detection and discrimination of individual pathogenic bacteria in a biological mixture. The light source can be modulated from 10 - 200 MHz and fluorescence emission is measured by a miniature photomultiplier tube after passing through a compact spectrometer. We have successfully extracted and measured the existence of a mixture of three far-red fluorescent dyes which have similar or overlapping spectral signatures. Data are presented demonstrating the instrument's ability to separate individual fluorophores from a complex mixture using a specially designed chemometric extraction algorithm. This instrument was designed for the possible direct detection and discrimination of the intrinsic fluorescence of biological warfare agents.

Citation Download Citation

Robert C. Chin, Michael W. Mayo, William Halliday, Noe Salazar, Clay Cruce, James P. Chambers, and James J. Valdes "Compact phase-resolved fluorescence spectrometer for detection and discrimination of airborne and waterborne bacteria", Proc. SPIE 2836, Chemical, Biochemical, and Environmental Fiber Sensors VIII, (10 December 1996); https://doi.org/10.1117/12.260589

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