Aggregation and hemolysis of human erythrocytes at photodynamic therapy

Alexander V. Priezzhev; Nikolai N. Firsov; Eugeny Ph. Stranadko; Olga M. Ryaboshapka; Tatyana V. Chichuk; O. V. Suschinskaya

doi:10.1117/12.260773

4 December 1996 Aggregation and hemolysis of human erythrocytes at photodynamic therapy

Alexander V. Priezzhev, Nikolai N. Firsov, Eugeny Ph. Stranadko, Olga M. Ryaboshapka, Tatyana V. Chichuk, O. V. Suschinskaya

Author Affiliations +

Proceedings Volume 2924, Photochemotherapy: Photodynamic Therapy and Other Modalities II; (1996) https://doi.org/10.1117/12.260773
Event: BiOS Europe '96, 1996, Vienna, Austria

Abstract

The goal of this work is to comparatively study the side effect PDT on blood with the use of two Russian photosensitizers -- photohem and photosense. These effects were studied at in vitro and in vivo conditions. The technique of backscattering nephelometry was used to measure the parameters of aggregation kinetics and the strength of aggregates of whole blood erythrocytes. The conventional method of absorption spectrophotometry was used to monitor the level of erythrocytes hemolysis. It was shown that sensitization of blood leads to the rise of the characteristic time of Rouleax formation, of the strength of aggregates, and of the level of hemolysis. The revealed tendency to increased strength of aggregates and level of hemolysis as a result of photodynamic action can be considered as a side effect of PDT.

Citation Download Citation

Alexander V. Priezzhev, Nikolai N. Firsov, Eugeny Ph. Stranadko, Olga M. Ryaboshapka, Tatyana V. Chichuk, and O. V. Suschinskaya "Aggregation and hemolysis of human erythrocytes at photodynamic therapy", Proc. SPIE 2924, Photochemotherapy: Photodynamic Therapy and Other Modalities II, (4 December 1996); https://doi.org/10.1117/12.260773

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available