4 December 1996 Combined treatment of photodynamic therapy and the topoisomerase I inhibitor camptothecin in growing V79 and NHIK 3025 cells
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Abstract
The topoisomerase I (Topo I) inhibitor camptothecin (CPT) has been combined with photodynamic treatment (PDT) in V79 and NHIK 3025 cells. Meso-tetra (N-methyl-4-pyridyl) porphine (TMPyPH2) was used as a photosensitizer. The dye has been shown to localize in granules in the cytoplasm of both cell lines. Some of the granularly located TMPyPH2 is relocated to the cytosol after exposure to a light dose inactivation 70% of the cells. The human NHIK 3025 carcinoma from cervix were more resistant to PDT (D50 approximately equals 0.33 J/cm2) and CPT (D50 approximately equals 320 nM) than Chinese hamster V79 lung fibroblasts (PDT, D20 approximately equals 0.3 J/cm2 and CPT, D20 approximately equals 55 nM). When the cells were treated with CPT for 18 hours before PDT, the combination of treatments led to slight synergistic effects for low CPT concentrations (up to 100 nM in NHIK 3025 cells) and high PDT doses (above 0.15 J/cm2). For higher CPT concentrations and lower PDT doses, the combination of treatment became additive.
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Jean-Michel Gaullier, Jean-Michel Gaullier, Siv Kjersti Rodal, Siv Kjersti Rodal, Johan Moan, Johan Moan, Kristian Berg, Kristian Berg, } "Combined treatment of photodynamic therapy and the topoisomerase I inhibitor camptothecin in growing V79 and NHIK 3025 cells", Proc. SPIE 2924, Photochemotherapy: Photodynamic Therapy and Other Modalities II, (4 December 1996); doi: 10.1117/12.260775; https://doi.org/10.1117/12.260775
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