You have requested a machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Neither SPIE nor the owners and publishers of the content make, and they explicitly disclaim, any express or implied representations or warranties of any kind, including, without limitation, representations and warranties as to the functionality of the translation feature or the accuracy or completeness of the translations.
Translations are not retained in our system. Your use of this feature and the translations is subject to all use restrictions contained in the Terms and Conditions of Use of the SPIE website.
4 December 1996Spectroscopic studies of photosensitizer-human serum albumin complexes and their photostability
The interaction of haematoporphyrin (Hp), dimethoxyhematoporphyrin IX (DMHp), meso-tetraphenylporphine tetrasulphonate (TPPS4), photofrin II (PF), chlorin e6 (Cle6) and aluminum phthalocyanine tetrasulphonate (AlPcS4) with human serum albumin (HSA) in aqueous solution and the influence of complexation on the photostability of sensitizers have been investigated by means of steady state absorption spectroscopy. By using the binding isotherm method and graphical Scatchard plot the sequence of affinity of sensitizers to HSA was obtained: TPPS4 greater than DMHp, Cle6, Hp, AlPcS4 greater than PF. The influence of aggregation on the binding ability of sensitizers is discussed. It was observed that complexation with HSA decreases the photostability of sensitizers. The increased rates of sensitizer photobleaching in the presence of photo-oxidizable substrates can be explained by the changes of photophysical properties of sensitizers caused by the interaction with substrates or by the attack of the biomolecules photo- oxidized by the type I mechanism involving electron transfer from excited porphyrin molecule or by the type II mechanism via singlet oxygen on porphyrin microcycle. The sequence of photostability of sensitizers in the presence and in the absence of HSA was established.
The alert did not successfully save. Please try again later.
A. Jasaitis Jr., Giedre Streckyte, Ricardas Rotomskis, "Spectroscopic studies of photosensitizer-human serum albumin complexes and their photostability," Proc. SPIE 2924, Photochemotherapy: Photodynamic Therapy and Other Modalities II, (4 December 1996); https://doi.org/10.1117/12.260770