6 December 1996 Depth-sensitive fluorescence detection of dyes in tissue phantoms
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It is well known that tumors can be detected by fluorescence imaging after administration of suitable photosensitizers. The tumor's thickness, however, an important diagnostic parameter, cannot be assessed by optical methods up to now. We show that depth resolution can be achieved in the case of a fluorescent dye distributed homogeneously in a superficial region of a light scattering medium. Diffusion theory shows that the ratio of the fluorescence measured at different illumination angles is over a wide range sensitive to the thickness of the dye containing layer only, regardless of the dye concentration. For the experimental proof, a new temporally and mechanically stable tissue phantom system was developed. The experimental results on tissue phantoms confirm the predictions of diffusion theory.
© (1996) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Gerd C. Beck, Willem M. Star, Hugo J. van Staveren, Lars H. P. Murrer, Angelika C. Rueck, Rudolf W. Steiner, "Depth-sensitive fluorescence detection of dyes in tissue phantoms", Proc. SPIE 2926, Optical Biopsies and Microscopic Techniques, (6 December 1996); doi: 10.1117/12.260822; https://doi.org/10.1117/12.260822

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