Fluorescent probes in biology and medicine

Jan Slavik

doi:10.1117/12.260826

6 December 1996 Fluorescent probes in biology and medicine

Jan Slavik

Proceedings Volume 2926, Optical Biopsies and Microscopic Techniques; (1996) https://doi.org/10.1117/12.260826
Event: BiOS Europe '96, 1996, Vienna, Austria

Abstract

The number of biological and medical applications of environmentally sensitive dyes -- fluorescent probes -- has been explosively increasing in the last few years. The application areas include mostly measurements of membrane viscosity, cytosol fluidity, membrane potential (distributive or electrochromic dyes) and mapping of intracellular ionic composition. Furthermore, fluorescent molecules or groups may specifically mark almost any type of binding site or any selected class of macromolecules. Nonfluorescent molecules such as enzyme substrates, lipids, nucleosides can be modified into fluorescent ones without substantially affecting their biological activity. The most promising field is the combination of fluorescent probes and fluorescence microscopy yielding time-resolved spectrally resolved two-dimensional or three-dimensional images.

Citation Download Citation

Jan Slavik "Fluorescent probes in biology and medicine", Proc. SPIE 2926, Optical Biopsies and Microscopic Techniques, (6 December 1996); https://doi.org/10.1117/12.260826

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available