Novel laser-based technology for cell separation

Edgar Diessel; Franco Fois; Ulrich Ebbinghaus-Kintscher; Juergen Stebani

doi:10.1117/12.260810

6 December 1996 Novel laser-based technology for cell separation

Edgar Diessel, Franco Fois, Ulrich Ebbinghaus-Kintscher, Juergen Stebani

Proceedings Volume 2926, Optical Biopsies and Microscopic Techniques; (1996) https://doi.org/10.1117/12.260810
Event: BiOS Europe '96, 1996, Vienna, Austria

Abstract

A UV laser-based separation technique for biological cells is presented. This method refers to the separation of cells that adhere to a special UV absorptive polymer foil. To separate a cell the UV microbeam excises a disc with the attached cell out of the foil. Then, the beam transfers this complex to a separate substrate. This principle allows a rapid separation of adherent cells without the risk of contamination. The operating conditions of the separation scheme were examined. The laser separation scheme was applied to the separation of human embryonic kidney (HEK- 293) cells. The viability tests revealed that these cells survived the procedure. Therefore, the laser separation method is considered a useful tool for separating biological cells.

Citation Download Citation

Edgar Diessel, Franco Fois, Ulrich Ebbinghaus-Kintscher, and Juergen Stebani "Novel laser-based technology for cell separation", Proc. SPIE 2926, Optical Biopsies and Microscopic Techniques, (6 December 1996); https://doi.org/10.1117/12.260810

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