6 December 1996 Novel laser-based technology for cell separation
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Abstract
A UV laser-based separation technique for biological cells is presented. This method refers to the separation of cells that adhere to a special UV absorptive polymer foil. To separate a cell the UV microbeam excises a disc with the attached cell out of the foil. Then, the beam transfers this complex to a separate substrate. This principle allows a rapid separation of adherent cells without the risk of contamination. The operating conditions of the separation scheme were examined. The laser separation scheme was applied to the separation of human embryonic kidney (HEK- 293) cells. The viability tests revealed that these cells survived the procedure. Therefore, the laser separation method is considered a useful tool for separating biological cells.
© (1996) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Edgar Diessel, Edgar Diessel, Franco Fois, Franco Fois, Ulrich Ebbinghaus-Kintscher, Ulrich Ebbinghaus-Kintscher, Juergen Stebani, Juergen Stebani, } "Novel laser-based technology for cell separation", Proc. SPIE 2926, Optical Biopsies and Microscopic Techniques, (6 December 1996); doi: 10.1117/12.260810; https://doi.org/10.1117/12.260810
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