Fiber optic approach to the fluorescence spectroscopy of cell cultures

Wolfgang Hoehne; Werner Schramm; V. Moritzen; U. Burgmann; Heinz Detlef Kronfeldt

doi:10.1117/12.260633

11 December 1996 Fiber optic approach to the fluorescence spectroscopy of cell cultures

Wolfgang Hoehne, Werner Schramm, V. Moritzen, U. Burgmann, Heinz Detlef Kronfeldt

Author Affiliations +

Proceedings Volume 2927, Optical and Imaging Techniques for Biomonitoring II; (1996) https://doi.org/10.1117/12.260633
Event: BiOS Europe '96, 1996, Vienna, Austria

Abstract

A fiber-optical spectrometer for pharmacologically oriented energy metabolism studies using NADH fluorescence has been built up. Starting from theoretical considerations, the system parameters have been optimized. With this optimized geometry, the sensitivity limit for quantitative NADH detection has reached 20 (mu) M. The absolute lower detectable concentration value limited by SNR and other background effects lies below 5 - 10 (mu) M. With cultures of endothelial cells, measurements of the age dependence of NADH fluorescence intensity have been performed. They give a criterion for the metabolic activity of cells selected for further pharmacological investigations.

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Wolfgang Hoehne, Werner Schramm, V. Moritzen, U. Burgmann, and Heinz Detlef Kronfeldt "Fiber optic approach to the fluorescence spectroscopy of cell cultures", Proc. SPIE 2927, Optical and Imaging Techniques for Biomonitoring II, (11 December 1996); https://doi.org/10.1117/12.260633

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