26 November 1996 Cellulose protein films for highly specific evanescent wave immunosensors
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Abstract
The immobilization techniques presented represent an innovative method for the highly specific determination of antigens and other proteins in a very short time and may be a useful tool for the industrial preparation of highly sensitive biosensors. Non amphiphilic alkylated cellulose films are used as matrices to reduce non-specific interactions with biomolecules. These films are transferred onto waveguides by using the Langmuir-Blodgett- technique. After photochemical stabilization the cellulose films serve as excellent matrices for the immobilization of proteins at high density. Furthermore, one-step immobilization of a mixed photopolymer antibody film with a continuously operating LB-trough is demonstrated. The activity and specificity of immobilized antibodies and streptavidin is controlled by an enzyme-linked immunosorbent assay (ELISA) and an evanescent wave immunosensor.
© (1996) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Frank Loescher, Frank Loescher, Andreas Hartmann, Andreas Hartmann, J. Ueberfeld, J. Ueberfeld, Thomas Ruckstuhl, Thomas Ruckstuhl, Daniel Bock, Daniel Bock, Thomas Jaworek, Thomas Jaworek, Gerhard Wegner, Gerhard Wegner, Stefan Seeger, Stefan Seeger, } "Cellulose protein films for highly specific evanescent wave immunosensors", Proc. SPIE 2928, Biomedical Systems and Technologies, (26 November 1996); doi: 10.1117/12.259974; https://doi.org/10.1117/12.259974
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