26 November 1996 Combined optical, electrostatic, and enzymatic handling of single DNA molecules
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Abstract
Complete handling of single DNA molecules and their enzymatic restriction are described. For that purpose a microsphere was bound to a DNA molecule and trapped by optical tweezers. It could be moved in any direction. For stretching or rotating the molecule an electrical field was applied while the bead was fixed by the optical tweezers. In a near-equilibrium state of the resulting forces, the DNA remained stretched. Subsequently, a restriction endonuclease was activated by liberating Mg2+ from a caged compound. The enzymatic reaction could be directly observed in the light microscope. While the bead remained in the focus of the laser trap the other restricted part of the DNA molecule is pulled away in the electrical field. The reaction was directly monitored and recorded on videotape.
© (1996) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Carsten Hoyer, Carsten Hoyer, Shamci Monajembashi, Shamci Monajembashi, Karl-Otto Greulich, Karl-Otto Greulich, } "Combined optical, electrostatic, and enzymatic handling of single DNA molecules", Proc. SPIE 2928, Biomedical Systems and Technologies, (26 November 1996); doi: 10.1117/12.259970; https://doi.org/10.1117/12.259970
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