Development of a versatile confocal Raman microscope

Robert D. Alcock; Mark Russell Dickinson; Terence A. King

doi:10.1117/12.259966

26 November 1996 Development of a versatile confocal Raman microscope

Robert D. Alcock, Mark Russell Dickinson, Terence A. King

Proceedings Volume 2928, Biomedical Systems and Technologies; (1996) https://doi.org/10.1117/12.259966
Event: BiOS Europe '96, 1996, Vienna, Austria

Abstract

A versatile confocal microscope has been developed that combines the optical sectioning properties of a confocal microscope with the chemical identification characteristics offered by Raman spectroscopy to yield a high performance imaging instrument. The apparatus is described and general alignment procedures discussed for optimum performance. An axial resolution of 1.8 micrometer has been determined experimentally for Raman active planar structure and its form compared to theory, A broad range of applications are envisaged in areas of biology, geology and other areas of microscopic analysis. A study is presented showing its applicability to photodynamic therapy for the localization of specific dyes within cells.

Citation Download Citation

Robert D. Alcock, Mark Russell Dickinson, and Terence A. King "Development of a versatile confocal Raman microscope", Proc. SPIE 2928, Biomedical Systems and Technologies, (26 November 1996); https://doi.org/10.1117/12.259966

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available