26 November 1996 Development of a versatile confocal Raman microscope
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Proceedings Volume 2928, Biomedical Systems and Technologies; (1996); doi: 10.1117/12.259966
Event: BiOS Europe '96, 1996, Vienna, Austria
Abstract
A versatile confocal microscope has been developed that combines the optical sectioning properties of a confocal microscope with the chemical identification characteristics offered by Raman spectroscopy to yield a high performance imaging instrument. The apparatus is described and general alignment procedures discussed for optimum performance. An axial resolution of 1.8 micrometer has been determined experimentally for Raman active planar structure and its form compared to theory, A broad range of applications are envisaged in areas of biology, geology and other areas of microscopic analysis. A study is presented showing its applicability to photodynamic therapy for the localization of specific dyes within cells.
© (1996) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Robert D. Alcock, Mark Russell Dickinson, Terence A. King, "Development of a versatile confocal Raman microscope", Proc. SPIE 2928, Biomedical Systems and Technologies, (26 November 1996); doi: 10.1117/12.259966; https://doi.org/10.1117/12.259966
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KEYWORDS
Raman spectroscopy

Microscopes

Confocal microscopy

Luminescence

Light scattering

Objectives

Spectroscopy

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