26 November 1996 Image analysis of cell natural fluorescence: diagnostic applications in haematology
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Abstract
Haematic cells, excited with radiation of suitable wavelength, give rise to a natural fluorescence (NF) emission. This paper investigates NF to develop new techniques for applications in both basic research and medical diagnostics. Results show that the cell populations examined exhibit peculiar emission bands. The intracellular fluorescence pattern reveals that flouresence is mainly located at cytoplasmic-level, thus related to the metabolic processes of the cells. The photophysical properties of cells appear different among the normal populations and between normal and leukaemic ones. Therefore the recognition of the various cellular elements, according to their fluorescence emission, is possible.
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Monica Monici, Monica Monici, Giovanni Agati, Giovanni Agati, Piero Mazzinghi, Piero Mazzinghi, Franco Fusi, Franco Fusi, Pietro Antonio Bernabei, Pietro Antonio Bernabei, Stefano Landini, Stefano Landini, Pierluigi Rossi Ferrini, Pierluigi Rossi Ferrini, Riccardo Pratesi, Riccardo Pratesi, } "Image analysis of cell natural fluorescence: diagnostic applications in haematology", Proc. SPIE 2928, Biomedical Systems and Technologies, (26 November 1996); doi: 10.1117/12.259969; https://doi.org/10.1117/12.259969
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