Optical real-time measurement of collagen denaturation

Vanitha Sankaran M.D.; Joseph T. Walsh Jr.

doi:10.1117/12.275490

16 June 1997 Optical real-time measurement of collagen denaturation

Vanitha Sankaran M.D., Joseph T. Walsh Jr.

Proceedings Volume 2975, Laser-Tissue Interaction VIII; (1997) https://doi.org/10.1117/12.275490
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

Linear birefringence is a property of collagenous tissue that results from both its composition and structure. Previous investigations have shown that birefringence provides an indication of structural changes in collagen during slow heating. We now report the birefringent response of both mature and young rat tail tendon to laser-heating. The results indicate that denaturation of collagen from mature rats induced by a 200-microsecond(s) -long Ho:YAG laser pulse may not be described accurately by kinetic parameters. Several second-long pulses of CO₂ laser pulse may not be described from young rats fit an Arrhenius model with E_a equals 12.1 kcal/mol and A equals e^18.03 s^-1. Typically, for slow-heating of collagen, E_a equals kcal/mol and A equals e¹²⁰ s^-1. Thus, it seems likely that the temperature and energy needed to initiate collagen denaturation is lower in young collagen, possibly due to its decreased hydroxyproline content and consequent decreased thermal stability.

Citation Download Citation

Vanitha Sankaran M.D. and Joseph T. Walsh Jr. "Optical real-time measurement of collagen denaturation", Proc. SPIE 2975, Laser-Tissue Interaction VIII, (16 June 1997); https://doi.org/10.1117/12.275490

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