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16 June 1997 Biomedical diagnostic with diffusely backscattered linearly and circularly polarized light
Andreas H. Hielscher; Angelia A. Eick; Judith R. Mourant; James P. Freyer; Irving J. Bigio
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Proceedings Volume 2976, Biomedical Sensing, Imaging, and Tracking Technologies II; (1997) https://doi.org/10.1117/12.275538
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States
Abstract
We report on the development of a method that records spatially dependent intensity patterns of polarized light that is diffusely backscattered from turbid materials. It is demonstrated that these intensity patterns can be used to differentiate between suspensions of cancerous and non- cancerous cells and to observe different metabolic cell processes.Our technique employs polarized light from a He-Ne laser, which is focused onto the surface of the scattering medium. A surface area of approximately 4 X 4 cm around the light input point is imaged through polarization- analysis optics onto a CCD camera. One can observe a manifold of intensity patterns by varying the polarization state of the incident laser light and changing the analyzer configuration to detect different polarization components of the backscattered light. Introducing the Mueller-matrix concept for diffusely backscattered light, a framework is provided to select a subset of measurements which comprehensively describe the optical properties of backscattering media.
© (1997) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
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Andreas H. Hielscher, Angelia A. Eick, Judith R. Mourant, James P. Freyer, and Irving J. Bigio "Biomedical diagnostic with diffusely backscattered linearly and circularly polarized light", Proc. SPIE 2976, Biomedical Sensing, Imaging, and Tracking Technologies II, (16 June 1997); https://doi.org/10.1117/12.275538
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