16 June 1997 Detection of fluorophore-labeled antibodies by surface-enhanced fluorescence on metal nanoislands
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Proceedings Volume 2976, Biomedical Sensing, Imaging, and Tracking Technologies II; (1997) https://doi.org/10.1117/12.275521
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States
Abstract
In fluorescence labelled immunosensing the discrimination of labels remaining in the bulk solution from labels bound to the analyte at the sensor surface is a basic optical problem. It is shown that application of surface enhanced fluorescence at a layer of noble metal nano-particles can increase the surface-to-background signal ratio. We explain the enhancement mechanism by an electrodynamic model and discuss the interaction between metal particle and fluorophore for the excitation and emission process. We show the principal guidelines for optimization of that processes. We find that the obtained discrimination power increases with decreasing intrinsic quantum efficiency of the fluorophore, suggesting the application of new classes of labels, namely low-quantum efficiency fluorophores. This theoretical finding is shown by a practical model experiment.
© (1997) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Thomas G. M. Schalkhammer, Thomas G. M. Schalkhammer, Franz Rembert Aussenegg, Franz Rembert Aussenegg, Alfred Leitner, Alfred Leitner, Harald Brunner, Harald Brunner, Gerhard Hawa, Gerhard Hawa, Christina Lobmaier, Christina Lobmaier, Fritz Pittner, Fritz Pittner, } "Detection of fluorophore-labeled antibodies by surface-enhanced fluorescence on metal nanoislands", Proc. SPIE 2976, Biomedical Sensing, Imaging, and Tracking Technologies II, (16 June 1997); doi: 10.1117/12.275521; https://doi.org/10.1117/12.275521
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