Cell growth assay using the ultrasonic flexural plate-wave device

Audra H. Meng; Richard M. White

doi:10.1117/12.269973

31 March 1997 Cell growth assay using the ultrasonic flexural plate-wave device

Audra H. Meng, Richard M. White

Proceedings Volume 2978, Micro- and Nanofabricated Electro-Optical Mechanical Systems for Biomedical and Environmental Applications; (1997) https://doi.org/10.1117/12.269973
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

The ability to easily monitor the rate of cell growth has many useful applications such as characterizing cell lines to optimize growth conditions and compound screening to test for cytotoxicity. As a cell growth sensor, the ultrasonic flexural plate-wave device offers a convenient assay that is non-invasive, continuous, and capable of full automation for high throughput screening. The flexural plate-wave (FPW) device is a microfabricated sensor consisting of a thin silicon nitride membrane with aluminum and zinc oxide transducers at both ends. These interdigitated transducers send and receive acoustic plate waves that propagate along the membrane. Applications of the device include mass sensing, achieved by measuring the frequency shift caused by mass loading of the membrane, and pumping of liquids and gases. We have conducted studies that established the biocompatibility of the FPW device materials with cell cultures. In addition, biological samples were exposed to the ultrasonic agitation of the device and no damaging effects were found. Experiments were performed using the FPW device to sense cell growth for mammalian suspension (non- adherent) cells. Results show a good correlation between change of cell number and frequency.

Citation Download Citation

Audra H. Meng and Richard M. White "Cell growth assay using the ultrasonic flexural plate-wave device", Proc. SPIE 2978, Micro- and Nanofabricated Electro-Optical Mechanical Systems for Biomedical and Environmental Applications, (31 March 1997); https://doi.org/10.1117/12.269973

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