Characterization of core-shell lattices by near-IR fluorescent cyanine dyes

Johannes W. Hofstraat; Gerrit D. B. van Houwelingen; Marcel J. Nuijens; Cees Gooijer; Nel H. Velthorst

doi:10.1117/12.273531

7 May 1997 Characterization of core-shell lattices by near-IR fluorescent cyanine dyes

Johannes W. Hofstraat, Gerrit D. B. van Houwelingen, Marcel J. Nuijens, Cees Gooijer, Nel H. Velthorst

Proceedings Volume 2980, Advances in Fluorescence Sensing Technology III; (1997) https://doi.org/10.1117/12.273531
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

Cyanine-based near-IR fluorescent probes have been applied as labels to determine aldehyde-groups in polymeric materials. The advantage of such probes is that they can be applied to polymers which are autofluorescent or strongly colored or to strongly scattering polymer emissions. The example which is discussed in this paper is the labelling of a polymeric core-shell latex, comprising a polystyrene core and a glycidylmethacrylate shell, which has been hydrolyzed and subsequently oxidized to form aldehyde functional groups, by application of a Cyanine with a hydrazide functionality. The Cyanine which has been applied absorbs and fluoresces in the 650 - 700 nm range. In addition to near-IR fluorescence, the Cyanine dyes have strong absorption (with extinction coefficients in the order of 2 X 10⁵ I/mol.cm). It is shown that by application of reflection absorption measurements also sensitive detection of labelled functional groups can be realized. The combined application of fluorescence and reflection measurements allows for the detection of concentration and distribution of functional groups in polymeric systems down to very low levels.

Citation Download Citation

Johannes W. Hofstraat, Gerrit D. B. van Houwelingen, Marcel J. Nuijens, Cees Gooijer, and Nel H. Velthorst "Characterization of core-shell lattices by near-IR fluorescent cyanine dyes", Proc. SPIE 2980, Advances in Fluorescence Sensing Technology III, (7 May 1997); https://doi.org/10.1117/12.273531

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available