Confocal Raman microscopic imaging of biological samples

Serguei Charonov; Igor Chourpa; Paolo Valisa; Michel Manfait

doi:10.1117/12.273519

7 May 1997 Confocal Raman microscopic imaging of biological samples

Serguei Charonov, Igor Chourpa, Paolo Valisa, Michel Manfait

Proceedings Volume 2980, Advances in Fluorescence Sensing Technology III; (1997) https://doi.org/10.1117/12.273519
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

Confocal Raman spectral imaging is discussed in terms of its application to studies within living cells. The main difficulty which arises, keeping in mind that cell viability should be respected, is the low sensitivity of Raman spectroscopy. We describe here some possible solutions to this problem: optimization of the instrumental conditions as well as use of surface-enhanced Raman scattering (SERS). SERS imaging is dependent on topology of the metal surface which is necessary to observe this effect. The advantages and limitations of different types of the SERS-active substrates when used for confocal Raman imaging of cells are analyzed using examples. We describe here the procedures and precautions we took when investigating metal-cell interfaces. The confocal Raman-SERS images presented demonstrate the applicability of SERS technique to selective analysis of intracellular distribution and molecular interactions of antitumor drugs.

Citation Download Citation

Serguei Charonov, Igor Chourpa, Paolo Valisa, and Michel Manfait "Confocal Raman microscopic imaging of biological samples", Proc. SPIE 2980, Advances in Fluorescence Sensing Technology III, (7 May 1997); https://doi.org/10.1117/12.273519

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