Detection of fluorescent and light-absorbing optical heterogeneity in tissue-mimicking phantoms using frequency-domain techniques

Guadiana Lopez; Tamara L. Troy; Jeffery S. Reynolds; Christina L. Hutchinson; Eva Marie Sevick-Muraca

doi:10.1117/12.273561

7 May 1997 Detection of fluorescent and light-absorbing optical heterogeneity in tissue-mimicking phantoms using frequency-domain techniques

Guadiana Lopez, Tamara L. Troy, Jeffery S. Reynolds, Christina L. Hutchinson, Eva Marie Sevick-Muraca

Author Affiliations +

Proceedings Volume 2980, Advances in Fluorescence Sensing Technology III; (1997) https://doi.org/10.1117/12.273561
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

Both theory and experimental results show the capacity to locate the presence of a fluorescent volume incurred within a scattering medium from frequency-domain measurements of photon migration. Frequency-domain measurements consisted of monitoring the phase-shift and amplitude demodulation of fluorescent and excitation light reemitted from a tissue phantom in response to a sinusoidally modulated excitation source located 2.8 cm away from a detecting fiber positioned on the phantom periphery. Measurements of modulation phase- shift and amplitude were conducted as a 9 mm diameter cylindrical heterogeneity containing micromolar concentrations of IR-125 and DTTCI in 0.5%. Intralipid was moved within a 20 cm diameter vessel filled with 0.5 Intralipid. These measurements were investigated to evaluate the contrast offered by lifetime, fluorescent yield, and the fluorophore concentration difference between the heterogeneity and its surroundings. Our results show that the degree of contrast offered by fluorescence is always superior to that afforded by light absorption, enabling better detection of a diseased tissue with preferential fluorescent dye uptake. Contrast is also enhanced by the lifetime of the fluorescent dye, its uptake into simulated diseased volume, and the volume into which preferential uptake occurs relative to the surrounding tissues.

Citation Download Citation

Guadiana Lopez, Tamara L. Troy, Jeffery S. Reynolds, Christina L. Hutchinson, and Eva Marie Sevick-Muraca "Detection of fluorescent and light-absorbing optical heterogeneity in tissue-mimicking phantoms using frequency-domain techniques", Proc. SPIE 2980, Advances in Fluorescence Sensing Technology III, (7 May 1997); https://doi.org/10.1117/12.273561

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
11 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Tissue optics

Demodulation

Tissues

Phase measurement

Absorption

Luminescence

Modulation

Show All Keywords

Keywords/Phrases

Search In:

Publication Years