7 May 1997 Fluorescent characteristics and pharmacokinetic profiles of the fluorescent probe BCECF in various tissues: the role of blood content
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Proceedings Volume 2980, Advances in Fluorescence Sensing Technology III; (1997); doi: 10.1117/12.273547
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States
Abstract
Microspectrofluorometry and fluorescence imaging have been largely used to measure the intracellular pH of living cells. Such in vivo measurements require the knowledge of the optical properties of the tissue and the pharmacokinetic of the dye. The purpose of this study was to investigate in vitro and in vivo spectral characteristics of BCECF. In vitro, measurements in presence of blood show that blood content can greatly affect pH measurement by increasing the fluorescence ratio. In vivo, the presence of blood in high vascularized tissue leads to the modification of BCECF spectral characteristics. Fluorescence kinetic profiles provides information about tissue perfusion. Consequently, pH measurements using BCECF or fluorescein derivatives by the double excitation method may be performed taking in account the tissue blood content and the tissue pharmacokinetic of the dye.
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Jean-Marie Devoisselle, Sylvie Soulie-Begu, Serge R. Mordon, H. Maillols, "Fluorescent characteristics and pharmacokinetic profiles of the fluorescent probe BCECF in various tissues: the role of blood content", Proc. SPIE 2980, Advances in Fluorescence Sensing Technology III, (7 May 1997); doi: 10.1117/12.273547; https://doi.org/10.1117/12.273547
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KEYWORDS
Luminescence

Blood

Tissues

Liver

In vivo imaging

Blood vessels

In vitro testing

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