7 May 1997 Heavy-atom modified near-IR fluorescent dyes for DNA sequencing applications: synthesis and photophysical characterization
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Proceedings Volume 2980, Advances in Fluorescence Sensing Technology III; (1997) https://doi.org/10.1117/12.273529
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States
Abstract
A series of near-IR fluorescent dyes have been prepared which contain an intramolecular heavy atom for altering the photophysics to produce a set of probes appropriate for single lane DNA sequencing applications. The identification of the terminal nucleotide base will be affected by temporal discrimination using fluorescence lifetime determination. The heavy-atom modification consists of an intramolecular halogen (mono- or disubstituted) situated on a remote section of the chromophore in order to minimize the perturbation on the photophysics. The series of dyes prepared showed similar absorption and emission maxima as well as fluorescence quantum yields that were similar. However, the lifetimes of these dyes were found to vary with the identity of the halogen substitution, yielding an apparent inverse heavy atom effect, with the heavier atom showing the longest fluorescence lifetime. Nanosecond flash photolysis spectroscopy of these dyes indicated that the intersystem crossing rates in the series increased with the heavier atom, consistent with known heavy-atom effects. The apparent inverse heavy atom effect resulted from decreases in the internal conversion rate of the base chromophore, with the heavier atom showing a smaller rate of internal conversion compared to that of the dye with the lighter heavy-atom modification.
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James H. Flanagan, Sarah E. Romero, Benjamin L. Legendre, Robert P. Hammer, Steven A. Soper, "Heavy-atom modified near-IR fluorescent dyes for DNA sequencing applications: synthesis and photophysical characterization", Proc. SPIE 2980, Advances in Fluorescence Sensing Technology III, (7 May 1997); doi: 10.1117/12.273529; https://doi.org/10.1117/12.273529
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