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7 May 1997 Imaging of fluorescence yield and lifetime from multiply scattered light re-emitted from random media
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Proceedings Volume 2980, Advances in Fluorescence Sensing Technology III; (1997)
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States
The feasibility of employing fluorescent agents to perform optical imaging in tissues and other scattering media has been examined through experimental and computational studies. Fluorescence lifetime and yield can give crucial information about local metabolite concentration or environmental conditions within tissues. This information can be employed towards disease detection, diagnosis, and treatment if non-invasively quantitated from re-emitted optical signals. However, the inverse problem for image reconstruction of fluorescence yield and lifetime is complicated due to the highly scattering nature of the tissue. In this work, a light propagation model employing the diffusion equation is used to account for the scattering of both the excitation and fluorescent light. Simulated measurements of frequency-domain parameters of fluorescent modulated AC amplitude and phase-lag are used as inputs to an inverse image reconstruction algorithm which employs the diffusion model to predict frequency-domain measurements resulting from a modulated input at the phantom periphery. In the inverse image reconstruction algorithm, we employ a Newton-Raphson technique combined with Marquardt algorithm to converge upon the fluorescent properties within the medium. The successful reconstruction of both the fluorescence yield and lifetime in the case of heterogeneous fluorophore distribution within a scattering medium has been demonstrated without a priori information or without the necessity of obtaining `absence' images.
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Eva Marie Sevick-Muraca and Dilip Y. Paithankar "Imaging of fluorescence yield and lifetime from multiply scattered light re-emitted from random media", Proc. SPIE 2980, Advances in Fluorescence Sensing Technology III, (7 May 1997);

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