Development of optical fiber sensor probes for rapid remote in situ spectroscopic measurements of biological samples

H. Georg Schulze; L. Shane Greek; Michael W. Blades; Alan V. Bree; Boris B. Gorzalka; Karl-Friedrich Klein; Robin F. B. Turner

doi:10.1117/12.273621

2 May 1997 Development of optical fiber sensor probes for rapid remote in-situ spectroscopic measurements of biological samples

H. Georg Schulze, L. Shane Greek, Michael W. Blades, Alan V. Bree, Boris B. Gorzalka, Karl-Friedrich Klein, Robin F. B. Turner

Author Affiliations +

Proceedings Volume 2982, Optical Diagnostics of Biological Fluids and Advanced Techniques in Analytical Cytology; (1997) https://doi.org/10.1117/12.273621
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

We have developed fiber-optic probes that facilitate rapid, simultaneous determination of multiple analytes, in situ, over a broad range of concentrations. Theoretical and empirical methods were used to design and characterize prototype probes that comprise a single small-diameter excitation fiber and multiple larger diameter collection fibers for the optical collection of side- and back- scattered or emitted light, depending on the sample characteristics. Prototype were developed for use with pulsed ultra-violet resonance Raman spectroscopy, however, probes of this type are also suitable for use with other spectroscopic techniques such as fluorescence. Materials specifications, modelling methods, fabrication methods, and performance characteristics are described. Probes of our design are at present capable of measuring the aromatic amino acids in the 10 (mu) M range and nM detection limits can be expected. We have also obtained UV Raman and resonance Raman spectra from proteins, DNA, amino acids, steroids, neurotransmitters, and alcohols with these probes.

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H. Georg Schulze, L. Shane Greek, Michael W. Blades, Alan V. Bree, Boris B. Gorzalka, Karl-Friedrich Klein, and Robin F. B. Turner "Development of optical fiber sensor probes for rapid remote in-situ spectroscopic measurements of biological samples", Proc. SPIE 2982, Optical Diagnostics of Biological Fluids and Advanced Techniques in Analytical Cytology, (2 May 1997); https://doi.org/10.1117/12.273621

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