Flow cytometers which incorporate light collection via a flow-stream waveguide

Raymond P. Mariella Jr.; Richard G. Langlois; Don A. Masquelier

doi:10.1117/12.273636

2 May 1997 Flow cytometers which incorporate light collection via a flow-stream waveguide

Raymond P. Mariella Jr., Richard G. Langlois, Don A. Masquelier

Author Affiliations +

Proceedings Volume 2982, Optical Diagnostics of Biological Fluids and Advanced Techniques in Analytical Cytology; (1997) https://doi.org/10.1117/12.273636
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

We have built several flow cytometers (FCMs) which incorporate the principle of light collection using a flow- stream waveguide (FSW). The smallest and most rugged version uses a diode laser emitting 820-nm light and collects perpendicular light scatter (PLS) with the FSW technique and collects forward light scatter (FLS) with a simple external lens. Both PLS and FLS detectors are silicon diodes with integral preamps; no fluorescence is collected. Two other units have used argon-ion lasers and a dichroic filter to separate PLS and fluorescence, and our studies have examined the factors which limit the performance of FSW light collection for low-level-fluorescence applications. In one case we have used a main-frame laser with line-tunable output and two cylindrical lenses for focusing onto the flow stream. This arrangement has allowed us to detect 93-nm yellow-green beads from Molecular Probes, Inc. and 140-nm green beads from Duke Scientific, Inc. A more recent 'luggable' version incorporates an air-cooled ion laser and a simple spherical lens for focusing the laser onto the flow stream. This FCM was able to detect the 93-nm beads from Molecular Probes. In all cases a flow stream in air is used as an optical waveguide that traps and transports light to one or more detectors.

Citation Download Citation

Raymond P. Mariella Jr., Richard G. Langlois, and Don A. Masquelier "Flow cytometers which incorporate light collection via a flow-stream waveguide", Proc. SPIE 2982, Optical Diagnostics of Biological Fluids and Advanced Techniques in Analytical Cytology, (2 May 1997); https://doi.org/10.1117/12.273636

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