Hemolysis of erythrocytes by amphiphiles: general regularities and membrane mechanisms

Gregory V. Kaler; Irene V. Yamaikina

doi:10.1117/12.273658

2 May 1997 Hemolysis of erythrocytes by amphiphiles: general regularities and membrane mechanisms

Gregory V. Kaler, Irene V. Yamaikina

Proceedings Volume 2982, Optical Diagnostics of Biological Fluids and Advanced Techniques in Analytical Cytology; (1997) https://doi.org/10.1117/12.273658
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

The time course of hemolysis by a number of amphiphiles differing in their molecular features was studied in isotonic solution under varying temperature and concentrations of amphiphile and erythrocytes. Some of detergents induced hemolysis with two-stage kinetics, the property not correlating with either the detergent polar group charge or critical micelle concentration. The first stage appears at relatively high amphiphile concentrations, starts without a notable lag-phase and stops within less than 1 min at an intermediate extent of lysis; the second stage has a distinct lag-phase followed by the exponential lysis phase always reaching complete hemolysis.The parameters of the two stages differently depend on temperature and concentration of sucrose. A general two- stage scheme of amphiphile-membrane interaction is proposed. During the former stage, amphiphile molecules, bound initially on the outer surface of the cell membrane, equilibrate across the membrane. At a sufficiently high amphiphile binding per cell, the first stage hemolysis occurs with the rate decreasing sharply in the course of transmembrane equilibration. After reaching the equilibrium distribution of detergent across membrane, hemolysis follows the second stage mechanism.

Citation Download Citation

Gregory V. Kaler and Irene V. Yamaikina "Hemolysis of erythrocytes by amphiphiles: general regularities and membrane mechanisms", Proc. SPIE 2982, Optical Diagnostics of Biological Fluids and Advanced Techniques in Analytical Cytology, (2 May 1997); https://doi.org/10.1117/12.273658

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available