Micromixing-stirring device for flow cytometry studies

Valeri V. Zenin; Nicolay D. Aksenov; Alla N. Shatrova; Nicolay V. Klopov; L. Scott Cram; Andrey I. Poletaev

doi:10.1117/12.273638

2 May 1997 Micromixing-stirring device for flow cytometry studies

Valeri V. Zenin, Nicolay D. Aksenov, Alla N. Shatrova, Nicolay V. Klopov, L. Scott Cram, Andrey I. Poletaev

Proceedings Volume 2982, Optical Diagnostics of Biological Fluids and Advanced Techniques in Analytical Cytology; (1997) https://doi.org/10.1117/12.273638
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

A special device able to perform fast mixing and stirring of samples with different reagents was placed inside the flow chamber of a flow cytometer. This mixing device is coupled with steppe-motor driven syringes which are able to inject desired volumes of sample and reagents under computer control. The fast mixing is performed by means of a small magnetic rod vibrating in alternating magnetic field. The device may work both in continuous and stepwise manner delivering defined volumes of sample and reagent at set time intervals. Internal volume of the mixing chamber is about 0.2 (mu) l. The frequency of stirring can vary up to 1 kHz thus providing different modes: mild mixing, breaking of cell aggregates, and cell-membrane rupture. This device was used for studies of DNA content distribution in cell cycle analysis, binding kinetics of DNA-specific dyes and for analysis of chromosome sets from prestained mitotic cells It is shown that the breaking of aggregates is essential for correct estimation of the S-phase. The most significant effects are observed in the case of activated lymphocytes and for the analysis of samples from solid tumors. Breaking of cell aggregates has been achieved by a simplified device placed just prior to the flow chamber.

Citation Download Citation

Valeri V. Zenin, Nicolay D. Aksenov, Alla N. Shatrova, Nicolay V. Klopov, L. Scott Cram, and Andrey I. Poletaev "Micromixing-stirring device for flow cytometry studies", Proc. SPIE 2982, Optical Diagnostics of Biological Fluids and Advanced Techniques in Analytical Cytology, (2 May 1997); https://doi.org/10.1117/12.273638

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available