Temperature dependence of erythrocyte aggregation in vitro by backscattering nephelometry

Igor V. Sirko; Nikolai N. Firsov; Olga M. Ryaboshapka; Alexander V. Priezzhev

doi:10.1117/12.273630

2 May 1997 Temperature dependence of erythrocyte aggregation in vitro by backscattering nephelometry

Igor V. Sirko, Nikolai N. Firsov, Olga M. Ryaboshapka, Alexander V. Priezzhev

Proceedings Volume 2982, Optical Diagnostics of Biological Fluids and Advanced Techniques in Analytical Cytology; (1997) https://doi.org/10.1117/12.273630
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

We apply backscattering nephelometry technique to register the alterations of the scattering signal from a whole blood sample due to appearance or disappearance of different types of erythrocyte aggregates in stasis and under controlled shear stress. The measured parameters are: the characteristic times of linear and 3D aggregates formation, and the strength of aggregates of different types. These parameters depend on the sample temperature in the range of 2 divided by 50 degrees C. Temporal parameters of the aggregation process strongly increase at temperature 45 degrees C. For samples of normal blood the aggregates strength parameters do not significantly depend on the sample temperature, whereas for blood samples from patients suffering Sjogren syndrome we observe high increase of the strength of 3D and linear aggregates and decrease of time of linear aggregates formation at low temperature of the sample. This combination of parameters is opposite to that observed in the samples of pathological blood at room temperature. Possible reasons of this behavior of aggregation state of blood and explanation of the observed effects will be discussed.

Citation Download Citation

Igor V. Sirko, Nikolai N. Firsov, Olga M. Ryaboshapka, and Alexander V. Priezzhev "Temperature dependence of erythrocyte aggregation in vitro by backscattering nephelometry", Proc. SPIE 2982, Optical Diagnostics of Biological Fluids and Advanced Techniques in Analytical Cytology, (2 May 1997); https://doi.org/10.1117/12.273630

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
5 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Blood

Backscatter

Temperature metrology

In vitro testing

Plasma

Proteins

Image analysis

Show All Keywords

Keywords/Phrases

Search In:

Publication Years