Use of CCD sensors in flow cytometry for nonimaging applications

Wolfgang Beisker

doi:10.1117/12.273645

2 May 1997 Use of CCD sensors in flow cytometry for nonimaging applications

Wolfgang Beisker

Proceedings Volume 2982, Optical Diagnostics of Biological Fluids and Advanced Techniques in Analytical Cytology; (1997) https://doi.org/10.1117/12.273645
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

The use of charge coupled devices (CCDs) as non-imaging sensors in flow cytometric systems to replace the classical photomultplier tubes (PMTs) is very advantageous: the quantum efficiency of the CCDs is about 5 to 10 times higher as for PMTs, the charge storage capability of CCDs avoids analogue processing of the fluorescence signals, the dynamic range is up to 18 bits and the fluorescence intensity at different wavelengths can be recorded on the same chip. In this report a full frame CCD imager is used in a thermoelectrically cooled environment. The output signal for the CCD is digitized with a 12-bit ADC and the data are sorted as list-mode data typically used in flow cytometric work. The performance of the system is demonstrated with DNA staining of mammalian cells with acridine-orange, propidium iodide and ethidium bromide. DNA histograms comparable with standard flow cytometry are recorded. From the same data set pulse-widths histograms can be processed and used for doublet discrimination. The high quantum efficiency of the CCD sensors is of special interest for fluorescing dyes in the dark red or near IR wavelength range.

Citation Download Citation

Wolfgang Beisker "Use of CCD sensors in flow cytometry for nonimaging applications", Proc. SPIE 2982, Optical Diagnostics of Biological Fluids and Advanced Techniques in Analytical Cytology, (2 May 1997); https://doi.org/10.1117/12.273645

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