23 May 1997 Multiuser facilities for microscopic imaging
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Proceedings Volume 2983, Functional Imaging and Optical Manipulation of Living Cells; (1997) https://doi.org/10.1117/12.274334
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States
Abstract
During the last ten years, microscopy as a scientific tool has reinvented itself. It has changed from a group of principally descriptive methodologies, to a wide range of primary tools and techniques to investigate the molecular organization of organs, tissues and cells. Advances in microscope and camera design, fluorescent dye technologies as well as the advent of inexpensive powerful computers, has made the simultaneous resolution and quantification of multiple concurrent molecular markers for both protein and DNA at a sub-micron resolution a reality. Furthermore, it is possible to probe living cells using a rapidly expanding repertoire of dyes sensitive to changes pH or concentration of specific intracellular ions. An essential extension of these techniques has been the development of methods which allow the distribution of proteins and mRNA to be studied within cells at a molecular resolution by EM. However, concurrent with these developments there has been a more dramatic increase in the level of expertise needed to successfully design experiments, implement these technologies and costs involved in purchasing and maintaining instrumentation. These developments have resulted in a situation such that it is no longer tenable for individual departments or possibly institutions to maintain independent imaging facilities, and centralized resources have become mandatory in order to stay at the cutting edge. This presentation will discuss the problems, pitfalls and advantages of a centralized system, and will focus on implementation, design, data handling, and management of a multi-user facility.
© (1997) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Simon Watkins, David S. Turner, "Multiuser facilities for microscopic imaging", Proc. SPIE 2983, Functional Imaging and Optical Manipulation of Living Cells, (23 May 1997); doi: 10.1117/12.274334; https://doi.org/10.1117/12.274334
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