23 May 1997 X-ray imaging of living cells
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Proceedings Volume 2983, Functional Imaging and Optical Manipulation of Living Cells; (1997) https://doi.org/10.1117/12.274326
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States
Abstract
X-ray images of living cells were obtained by using a flash contact x-ray microscope system. The system consists of a high power laser (grass laser or KrF laser) for producing a plasma as an x-ray source, a small vacuum chamber, and a sample holder with a metal target for making plasma. The x- ray images were recorded on an x-ray sensitive layer on a silicon wafer. After chemical development x-ray images on the x-ray sensitive layers were read out by using an atomic force microscope. The performance of the system is demonstrated by presenting x-ray images of unicellular microorganisms, bacteria and skeletal muscle fibers. The intracellular structures were visible in some cases. For laboratory use a flash contact table-top x-ray microscopy is improved using a table-scaled YAG laser. The table-top systems showed good performance comparing to that of the x- ray microscope system with high power laser. These results indicate that the table-top x-ray microscope is a powerful instrumentation for the observation of living cells.
© (1997) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Toshikazu Majima, Toshikazu Majima, Hideaki Shimizu, Hideaki Shimizu, Toshihisa Tomie, Toshihisa Tomie, Eisuke Miura, Eisuke Miura, Toshihiko Kanayama, Toshihiko Kanayama, Masahiro Yamada, Masahiro Yamada, "X-ray imaging of living cells", Proc. SPIE 2983, Functional Imaging and Optical Manipulation of Living Cells, (23 May 1997); doi: 10.1117/12.274326; https://doi.org/10.1117/12.274326
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