Comparison of experiment and theory for finite-sized multiple-aperture arrays in direct-view microscopy

Eithne M. McCabe; D. T. Fewer; Simon J. Hewlett

doi:10.1117/12.271255

10 April 1997 Comparison of experiment and theory for finite-sized multiple-aperture arrays in direct-view microscopy

Eithne M. McCabe, D. T. Fewer, Simon J. Hewlett

Author Affiliations +

Proceedings Volume 2984, Three-Dimensional Microscopy: Image Acquisition and Processing IV; (1997) https://doi.org/10.1117/12.271255
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

Experimental results are given for the optical sectioning characteristics of finite-sized, multiple-aperture arrays in brightfield direct-view microscopy. We also present a theoretical model for the brightfield direct-view microscope (DVM). This allows us to determine the optical sectioning strength for finite-sized, multiple-pinhole arrays with an arbitrary distribution of apertures. The theoretical model is modified, for experimental purposes, to take account of the presence of objective lens pupil shading. A comparison between experimental and theoretical results for the axial response of the DVM to a plane mirror specimen is presented. In particular, the effects of pinhole size, pinhole spacing and array geometry are investigated in detail with a veil to (1) achieving good optical sectioning characteristics and (2) maximizing the amount of light available for imaging. The implications of our results for practical systems as regards pinhole-array design and fabrication are also discussed.

Citation Download Citation

Eithne M. McCabe, D. T. Fewer, and Simon J. Hewlett "Comparison of experiment and theory for finite-sized multiple-aperture arrays in direct-view microscopy", Proc. SPIE 2984, Three-Dimensional Microscopy: Image Acquisition and Processing IV, (10 April 1997); https://doi.org/10.1117/12.271255

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