Improved resolution in PSAF imaging

Michiel Mueller; G. J. Brakenhoff

doi:10.1117/12.271269

10 April 1997 Improved resolution in PSAF imaging

Michiel Mueller, G. J. Brakenhoff

Proceedings Volume 2984, Three-Dimensional Microscopy: Image Acquisition and Processing IV; (1997) https://doi.org/10.1117/12.271269
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

In PSAF (point spread autocorrelation function) imaging a fluorescence signal is generated from the interference response in the overlap region of two spatially shifted point spread functions (PSFs). It is experimentally demonstrated that a resolution improvement of approximately 30% can be realized in the case of axially shifted PSFs under high numerical-aperture (NA) conditions. A similar improvement in resolution is expected from numerical modeling for the lateral case. The presented technique can also be applied to the measurement of the effective point spread function itself in all three dimensions. It is found that the technique -- which in the latter case uses a bulk fluorescing solution -- is an excellent tool to access the apodization conditions of a practical optical system, such as a high-numerical aperture objective.

Citation Download Citation

Michiel Mueller and G. J. Brakenhoff "Improved resolution in PSAF imaging", Proc. SPIE 2984, Three-Dimensional Microscopy: Image Acquisition and Processing IV, (10 April 1997); https://doi.org/10.1117/12.271269

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available