10 April 1997 Virus structure using the computer-aided phase microscope Airyscan
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Proceedings Volume 2984, Three-Dimensional Microscopy: Image Acquisition and Processing IV; (1997); doi: 10.1117/12.271268
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States
Abstract
Investigation of features and functions of some small biological objects (smaller than 500 nm), in particular, viruses, with conventional optical microscopy is practically impossible. Usually their images are obtained with methods of scanning electron microscopy (SEM), which precludes work with samples in a native state. We obtained images of different viruses including influenza A virus in native state with computer-aided phase microscope (CPM) Airyscan, in which an He-Ne laser is used as a light source. The main purpose of this work was to show the possibility to obtain adequate structure images of influenza viruses with diameter about 100 nm in conditions quite close to native and to investigate different stages of influenza virus budding. We suppose that these results may be considered as a basis for further studies of cell-virus interaction.
© (1997) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Vladimir P. Tychinsky, Nikolai V. Kaverin, Elena V. Perevedentseva, Tatiana V. Vyshenskaia, Georgy E. Kufal, "Virus structure using the computer-aided phase microscope Airyscan", Proc. SPIE 2984, Three-Dimensional Microscopy: Image Acquisition and Processing IV, (10 April 1997); doi: 10.1117/12.271268; https://doi.org/10.1117/12.271268
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KEYWORDS
Microscopes

Viruses

Scanning electron microscopy

Helium neon lasers

Light sources

Microscopy

Basic research

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