22 May 1997 All-solid-state TCPC instrument for dynamic lifetime measurements in sensitive DNA analysis
Author Affiliations +
Proceedings Volume 2985, Ultrasensitive Biochemical Diagnostics II; (1997) https://doi.org/10.1117/12.274364
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States
Abstract
We have constructed a compact, all solid state, time correlated single photon counting device for sensitive measurement of the time resolved behavior of DNA fragments passing through a capillary tube. The instrument consists of a pulsed diode laser PDL, a single photon avalanche diode SPCM-211 and a PC board containing all TCPC electronics. We achieved an overall instrument response function of the system of less than 300 ps. This is fast enough for measurement of fluorescence lifetimes. We demonstrated the utility and the sensitivity of the instrument; dynamic measurements of fluorescence decays for NIR dye-labelled nucleotide bases were measured during capillary electrophoresis. The results indicate that in a two-dye experiment the characteristic lifetime of the probe could be used to identify the terminal nucleotide base. The lifetimes of the tow dyes bound to DNA fragments were determined to be 670 ps and 530 ps.
© (1997) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Rainer Erdmann, Rainer Erdmann, Michael Wahl, Michael Wahl, Kristian Lauritsen, Kristian Lauritsen, Joerg Enderlein, Joerg Enderlein, Benjamin L. Legendre, Benjamin L. Legendre, Daryl C. Williams, Daryl C. Williams, Steven A. Soper, Steven A. Soper, } "All-solid-state TCPC instrument for dynamic lifetime measurements in sensitive DNA analysis", Proc. SPIE 2985, Ultrasensitive Biochemical Diagnostics II, (22 May 1997); doi: 10.1117/12.274364; https://doi.org/10.1117/12.274364
PROCEEDINGS
7 PAGES


SHARE
Back to Top