Wide-field imaging design for a multiple-capillary DNA-sequencing system

Lyle M. Nay; Robert Sinclair; Harold Swerdlow

doi:10.1117/12.274354

22 May 1997 Wide-field imaging design for a multiple-capillary DNA-sequencing system

Lyle M. Nay, Robert Sinclair, Harold Swerdlow

Proceedings Volume 2985, Ultrasensitive Biochemical Diagnostics II; (1997) https://doi.org/10.1117/12.274354
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

A laser-induced fluorescence detection system compatible with a capillary electrophoresis array was developed. The design incorporates fiber-optic excitation and a detection system including a diffraction grating and a CCD camera. The system employs no moving parts and is capable of producing data comparable to commercially available systems. It is based on a spectrally-resolved four-dye sequencing scheme. The conceptual design was proven, however, refinements must be made to optimize performance for high-throughput capillary-array DNA sequencing. Automated sample preparation and loading in combination with a refillable separation- matrix capillary-array system could prove to be an invaluable tool for completion of the Human Genome Project.

Citation Download Citation

Lyle M. Nay, Robert Sinclair, and Harold Swerdlow "Wide-field imaging design for a multiple-capillary DNA-sequencing system", Proc. SPIE 2985, Ultrasensitive Biochemical Diagnostics II, (22 May 1997); https://doi.org/10.1117/12.274354

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