Translator Disclaimer
13 February 1997 Simple method of enzyme immobilization for pH-ISFET-based urea biosensors
Author Affiliations +
Proceedings Volume 3054, Optoelectronic and Electronic Sensors II; (1997) https://doi.org/10.1117/12.266713
Event: Optoelectronic and Electronic Sensors II, 1996, Szczyrk, Poland
Abstract
In this paper, a simple chemical method of urease immobilization on silicon nitride surface is described. As a basic structure to construct urea-biosensor, a pH-sensitive Si3N4-gate ISFET was used. The developed method of chemical immobilization of urease is based on Schiff's base formation. The developed EnFET type urea biosensor are characterized by the following parameters: (1) maximum analytical signal: 120 divided by 140 mV in 10 mM phosphate buffer solution, (2) linear range of the (Delta) Ugs equals f(logCurea): pCurea(2 divided by 3.5) in 10 mM phosphate buffer, (3) response time: 80 s and (4) lifetime: 35 days with the stable analytical signal then after 52 days this signal decreased by at least 40%. The influence of the concentration as well as the pH of the buffer solution on EnFET response were investigated.
© (1997) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Dorota Pijanowska and Wladislaw Torbicz "Simple method of enzyme immobilization for pH-ISFET-based urea biosensors", Proc. SPIE 3054, Optoelectronic and Electronic Sensors II, (13 February 1997); https://doi.org/10.1117/12.266713
PROCEEDINGS
8 PAGES


SHARE
Advertisement
Advertisement
Back to Top