Translator Disclaimer
11 July 1997 Activities of microorganisms and enzymes in water-restricted environments: biological activities in aqueous compartments at micron scale
Author Affiliations +
Abstract
In water-in-oil microemulsions, microdroplets of water, surrounded by a layer of surfactant molecules (reversed micelles), are dispersed in an organic solvent. Various microorganisms (unicellular algae and cyanobacteria) and isolated enzymes were dispersed in microemulsions without loss of biological activity. Each biological system needed a defined quantity of water in the microemulsion for maximum activity. Under optimum conditions, microbial enzymes for various sources (hydrogenases, dehydrogenases) exhibited, besides ten-fold increase in specific activity, a temperature optimum up to 16 degree(s)C higher as compared to aqueous solutions. These experimental findings, together with theoretical considerations, imply that water structure inside reversed micelles is very different from free water, but similar to water in narrow compartments with polar or ionic surfaces. These compartments may represent a model system for environments, where (liquid) water is not available in bulk amounts, but embedded in an anhydrous matrix.
© (1997) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Michael Hoppert, Klaus Mlejnek, Beatrix Seiffert, and Frank Mayer "Activities of microorganisms and enzymes in water-restricted environments: biological activities in aqueous compartments at micron scale", Proc. SPIE 3111, Instruments, Methods, and Missions for the Investigation of Extraterrestrial Microorganisms, (11 July 1997); https://doi.org/10.1117/12.278806
PROCEEDINGS
9 PAGES


SHARE
Advertisement
Advertisement
RELATED CONTENT

A new diagnostic device: KINOX
Proceedings of SPIE (December 06 2006)
Life in ice: implications to astrobiology
Proceedings of SPIE (September 11 2009)
RNA World meets Snowball Earth
Proceedings of SPIE (September 07 2010)

Back to Top