Picosecond infrared studies of protein vibrational modes: the amide I mode of myoglobin

Kristen A. Peterson; James R. Engholm; Chris W. Rella; H. Alan Schwettman

doi:10.1117/12.290259

16 October 1997 Picosecond infrared studies of protein vibrational modes: the amide I mode of myoglobin

Kristen A. Peterson, James R. Engholm, Chris W. Rella, H. Alan Schwettman

Proceedings Volume 3153, Accelerator-Based Infrared Sources and Applications; (1997) https://doi.org/10.1117/12.290259
Event: Optical Science, Engineering and Instrumentation '97, 1997, San Diego, CA, United States

Abstract

The population lifetime of the amide I vibration (v₁₀ fundamental, ca. 1650 cm^-1) in the protein myoglobin in D₂O has been determined by picosecond infrared pump- probe spectroscopy using the Stanford mid-infrared free electron laser to be 1.3 +/- 0.2 ps. In a glass forming mixture of deuterated glycerol and D₂O, the vibrational lifetime was found to increase from 1.3 +/- 0.2 ps at 310 K to 1.8 +/- 0.2 ps at 10 K. In addition to determining the time-scale of vibrational relaxation, we also observed multi-level vibrational excitation which has implications regarding the anharmonicity and homogeneous linewidth of the mode.

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Kristen A. Peterson, James R. Engholm, Chris W. Rella, and H. Alan Schwettman "Picosecond infrared studies of protein vibrational modes: the amide I mode of myoglobin", Proc. SPIE 3153, Accelerator-Based Infrared Sources and Applications, (16 October 1997); https://doi.org/10.1117/12.290259

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