16 October 1997 Picosecond infrared studies of protein vibrational modes: the amide I mode of myoglobin
Author Affiliations +
Abstract
The population lifetime of the amide I vibration (v10 fundamental, ca. 1650 cm-1) in the protein myoglobin in D2O has been determined by picosecond infrared pump- probe spectroscopy using the Stanford mid-infrared free electron laser to be 1.3 +/- 0.2 ps. In a glass forming mixture of deuterated glycerol and D2O, the vibrational lifetime was found to increase from 1.3 +/- 0.2 ps at 310 K to 1.8 +/- 0.2 ps at 10 K. In addition to determining the time-scale of vibrational relaxation, we also observed multi-level vibrational excitation which has implications regarding the anharmonicity and homogeneous linewidth of the mode.
© (1997) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Kristen A. Peterson, Kristen A. Peterson, James R. Engholm, James R. Engholm, Chris W. Rella, Chris W. Rella, H. Alan Schwettman, H. Alan Schwettman, } "Picosecond infrared studies of protein vibrational modes: the amide I mode of myoglobin", Proc. SPIE 3153, Accelerator-Based Infrared Sources and Applications, (16 October 1997); doi: 10.1117/12.290259; https://doi.org/10.1117/12.290259
PROCEEDINGS
10 PAGES


SHARE
Back to Top