16 October 1997 Synchrotron infrared microspectroscopy as a means of studying the chemical composition of bone: applications to osteoarthritis
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Abstract
Infrared microspectroscopy combines microscopy and spectroscopy for the purpose of chemical microanalysis. Light microscopy provides a way to generate and record magnified images and visibly resolve microstructural detail. Infrared spectroscopy provides a means for analyzing the chemical makeup of materials. Combining light microscopy and infrared spectroscopy permits the correlation of microstructure with chemical composition. Inherently, the long wavelengths of infrared radiation limit the spatial resolution of the technique. However, synchrotron infrared radiation significantly improves both the spectral and spatial resolution of an infrared microspectrometer, such that data can be obtained with high signal-to-noise at the diffraction limit, which is 3 - 5 micrometers in the mid-infrared region.
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Lisa M. Miller, Cathy S. Carlson, G. Lawrence Carr, Gwyn P. Williams, Mark R. Chance, "Synchrotron infrared microspectroscopy as a means of studying the chemical composition of bone: applications to osteoarthritis", Proc. SPIE 3153, Accelerator-Based Infrared Sources and Applications, (16 October 1997); doi: 10.1117/12.290258; https://doi.org/10.1117/12.290258
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