X-ray irradiation of yeast cells

Alessandra Masini; Dimitri Batani; Fabio Previdi; Aldo Conti; Francesca Pisani; Cesare Botto; Fulvia Bortolotto; Flavia Torsiello; I. C. Edmond Turcu; Ric M. Allott; Nicola Lisi; Marziale Milani; Michele Costato; Achille Pozzi; Michel Koenig

doi:10.1117/12.283997

14 October 1997 X-ray irradiation of yeast cells

Alessandra Masini, Dimitri Batani, Fabio Previdi, Aldo Conti, Francesca Pisani, Cesare Botto, Fulvia Bortolotto, Flavia Torsiello, I. C. Edmond Turcu, Ric M. Allott, Nicola Lisi, Marziale Milani, Michele Costato, Achille Pozzi, Michel Koenig

Author Affiliations +

Proceedings Volume 3157, Applications of X Rays Generated from Lasers and Other Bright Sources; (1997) https://doi.org/10.1117/12.283997
Event: Optical Science, Engineering and Instrumentation '97, 1997, San Diego, CA, United States

Abstract

Saccharomyces Cerevisiae yeast cells were irradiated using the soft X-ray laser-plasma source at Rutherford Laboratory. The aim was to produce a selective damage of enzyme metabolic activity at the wall and membrane level (responsible for fermentation) without interfering with respiration (taking place in mitochondria) and with nuclear and DNA activity. The source was calibrated by PIN diodes and X-ray spectrometers. Teflon stripes were chosen as targets for the UV laser, emitting X-rays at about 0.9 keV, characterized by a very large decay exponent in biological matter. X-ray doses to the different cell compartments were calculated following a Lambert-Bouguet-Beer law. After irradiation, the selective damage to metabolic activity at the membrane level was measured by monitoring CO₂ production with pressure silicon detectors. Preliminary results gave evidence of pressure reduction for irradiated samples and non-linear response to doses. Also metabolic oscillations were evidenced in cell suspensions and it was shown that X-ray irradiation changed the oscillation frequency.

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Alessandra Masini, Dimitri Batani, Fabio Previdi, Aldo Conti, Francesca Pisani, Cesare Botto, Fulvia Bortolotto, Flavia Torsiello, I. C. Edmond Turcu, Ric M. Allott, Nicola Lisi, Marziale Milani, Michele Costato, Achille Pozzi, and Michel Koenig "X-ray irradiation of yeast cells", Proc. SPIE 3157, Applications of X Rays Generated from Lasers and Other Bright Sources, (14 October 1997); https://doi.org/10.1117/12.283997

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